Signalling

Part:BBa_K1369003:Design

Designed by: Andrew Hou   Group: iGEM14_Caltech   (2014-09-04)


AgrC+4xSH3 domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 68
    Illegal BglII site found at 438
    Illegal BamHI site found at 2186
    Illegal XhoI site found at 2195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1481
    Illegal SapI.rc site found at 1691
    Illegal SapI.rc site found at 1901
    Illegal SapI.rc site found at 2111


Design Notes

AgrC is a transmembrane histidine kinase. Because the 4xSH3 domain is part of a scaffold system that increases binding affinity between AgrC and the response regulator AgrA, it is necessary that the 4xSH3 domain be attached to the C-terminus, which faces the inside of the cell.


Source

AgrC is endogenous to Staphylococcus aureus, and is a histidine kinase receptor involved in the AgrBDCA quorum sensing system.

References